fluorescent dna binding agent  (Thermo Fisher)

Journal: Frontiers in Pediatrics

Article Title: Exposure to Morphine and Caffeine Induces Apoptosis and Mitochondrial Dysfunction in a Neonatal Rat Brain

doi: 10.3389/fped.2020.00593

Figure Lengend Snippet: DNA Damage: Representative fluorescence microscopy images of brain tissue sections of Control, MOR, CAFF, and M+C treated pups at PND 7, PND 14, and PND 28 and a graph of quantified 7AAD + cells. Scale bar = 100 μm. Values are expressed as mean ± S.E.M. * P < 0.001 vs. Control.

Article Snippet: The brain sections were incubated with fluorescent DNA binding agent, 7AAD (0.25 μg/mL; Thermo Fisher Scientific, Bartlett, IL) for 15 min at 4°C.

Techniques: Fluorescence, Microscopy, Control

Journal: Frontiers in Pediatrics

Article Title: Exposure to Morphine and Caffeine Induces Apoptosis and Mitochondrial Dysfunction in a Neonatal Rat Brain

doi: 10.3389/fped.2020.00593

Figure Lengend Snippet:

Article Snippet: The brain sections were incubated with fluorescent DNA binding agent, 7AAD (0.25 μg/mL; Thermo Fisher Scientific, Bartlett, IL) for 15 min at 4°C.

Techniques: Saline